The final predicted expression of both CBS OPT and L2HGDH OPT were MYPYDVPDYALQISCBS OPT (or L2HGDH OPT )DYKDDDDKKSRGPGRATGAAANSAVDGTAGPGSTGSRVDGSGEGRGSLLTCGDVEENPGP (with the features: HA tag, amino acid linker 1, CBS OPT (or L2HGDH OPT ), FLAG, amino acid linker 2, T2A self-cleavage site, in the order of N to C terminal) (#230088 and #230089, Addgene)
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Cysteine availability usually limits how much GSH you can make
S., Sinclair, C., Johnson, B., Peter, A., Taylor, M., Hettrick, L., Chapman, M., Klein, S., Solanki, A., Gattis, D., Watt, A., Hughes, A
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